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3D‐reconstruction and functional properties of GFP‐positive and GFP‐negative granule cells in the fascia dentata of the Thy1‐GFP mouse by Mario Vuksic; Domenico Del Turco; Carlos Bas Orth; Guido J. Burbach; Guoping Feng; Christian M. Müller; Stephan W. Schwarzacher; Thomas Deller is a Neuroscience article available to read on EtoBox.
What is 3D‐reconstruction and functional properties of GFP‐positive and GFP‐negative granule cells in the fascia dentata of the Thy1‐GFP mouse about?
## Abstract Granule cells of the mouse fascia dentata are widely used in studies on neuronal development and plasticity. In contrast to the rat, however, high‐resolution morphometric data on these cells are scarce. Thus, we have analyzed granule cells in the fascia dentata of the adult Thy1‐GFP mouse (C57BL/6 background). In this mouse line, single neurons in the granule cell layer are GFP‐labeled, making them amenable to high‐resolution 3D‐reconstruction. First, calbindin or parvalbumin‐immunofluorescence was used to identify GFP‐positive cells as granule cells. Second, the dorsal‐ventral distribution of GFP‐positive granule cells was studied: In the dorsal part of the fascia dentata 11% and in the ventral part 15% of all granule cells were GFP‐positive. Third, GFP‐positive and GFP‐negative granule cells were compared using intracellular dye‐filling (fixed slice technique) and patch‐clamp recordings; no differences were observed between the cells. Finally, GFP‐positive granule cells (dorsal and ventral fascia dentata) were imaged at high resolution with a confocal microscope, 3D‐reconstructed in their entirety and analyzed for soma size, total dendritic length, number of segments,
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- Author
- Mario Vuksic; Domenico Del Turco; Carlos Bas Orth; Guido J. Burbach; Guoping Feng; Christian M. Müller; Stephan W. Schwarzacher; Thomas Deller
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1050-9631)
- Published
- 2008
- Language
- EN
- Field
- Neuroscience (Life Sciences)