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Antibody–drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells by Muhammad Raisul Abedin; Kaitlyne Powers; Rachel Aiardo; Dibbya Barua; Sutapa Barua is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Antibody–drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells about?
Chemotherapeutic drugs suffer from non-specific binding, undesired toxicity, and poor blood circulation which contribute to poor therapeutic efficacy. In this study, antibody–drug nanoparticles (ADNs) are engineered by synthesizing pure anti-cancer drug nanorods (NRs) in the core of nanoparticles with a therapeutic monoclonal antibody, Trastuzumab on the surface of NRs for specific targeting and synergistic treatments of human epidermal growth factor receptor 2 (HER2) positive breast cancer cells. ADNs were designed by first synthesizing ~ 95 nm diameter × ~ 500 nm long paclitaxel (PTX) NRs using the nanoprecipitation method. The surface of PTXNRs was functionalized at 2′ OH nucleophilic site using carbonyldiimidazole and conjugated to TTZ through the lysine residue interaction forming PTXNR-TTZ conjugates (ADNs). The size, shape, and surface charge of ADNs were characterized using scanning electron microscopy (SEM), SEM, and zeta potential, respectively. Using fluorophore labeling and response surface analysis, the percentage conjugation efficiency was found > 95% with a PTX to TTZ mass ratio of 4 (molar ratio ≈ 682). In vitro therapeutic efficiency of PTXNR-TTZ was evaluated in t
Who reads Antibody–drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Muhammad Raisul Abedin; Kaitlyne Powers; Rachel Aiardo; Dibbya Barua; Sutapa Barua
- Publisher
- Springer Science and Business Media LLC
- Published
- 2021
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)