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Can I read Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification on EtoBox?
Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification by Ermias Melles; Henrik Anderson; Daniel Wallinder; Jawed Shafqat; Tomas Bergman; Teodor Aastrup; Hans Jörnvall is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification about?
Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous Xow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for eYcient recovery and mass spectrometric identiWcation. The method is reproducible through repeated cycles, providing aYnity puriWcation of proteins under real-time monitoring of the binding and elution processes.
Who reads Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Ermias Melles; Henrik Anderson; Daniel Wallinder; Jawed Shafqat; Tomas Bergman; Teodor Aastrup; Hans Jörnvall
- Publisher
- Elsevier Science; Elsevier ; Elsevier Inc.; Elsevier BV (ISSN 0003-2697)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)