About this document
Immunohistochemistry Techniques Overview by Amita Negi is a document available to read on EtoBox.
Immuno histochemistry (IHC) is a technique that identifies cellular or tissue antigens through antigen-antibody binding, which can be visualized using markers like fluorescent dyes, enzymes, or metals. The document discusses the principles, protocol, controls, and troubleshooting of IHC. It describes antigen retrieval, primary and secondary antibody incubation, blocking steps, immunolabeling methods, chromogens, and nuclear counterstaining used in IHC.
- Author
- Amita Negi
- Language
- EN