About this Neuroscience article

A Novel Subtype of Prostacyclin Receptor in the Central Nervous System by Yumiko Watanabe; Kiyoshi Matsumura; Hajime Takechi; Koichi Kato; Hiroshi Morii; Margareta Björkman; Bengt Langstrom; Ryoji Noyori; Masaaki Suzuki; Yasuyoshi Watanabe is a Neuroscience article available to read on EtoBox.

Abstract: Recently, in the course of our search for the prostacyclin receptor in the brain, we found a novel subtype, designated as IP~2~, which was finely discriminated by use of the specific ligand (15__R__)‐16‐__m__‐tolyl‐17,18,19,20‐tetranorisocarbacyclin (15__R__‐TIC) and specifically localized in the rostral part of the brain. In the present study, the tritiated compound 15__R__‐[15‐^3^H]TIC was synthesized and utilized for more specific research on IP~2~. The specificity of binding to rat brain regions was confirmed by use of several prostacyclin derivatives including 15__S__‐TIC. Mapping of 15__R__‐ and 15__S__‐[^3^H]TIC binding in adjacent pairs of frozen sections of rat brain demonstrated a quite similar pattern of distribution in almost all rostral brain regions, indicating that the regions may contain only the IP~2~ subtype. On the other hand, 15__R__‐[^3^H]TIC binding was very faint as compared with 15__S__‐[^3^H]TIC binding in the caudal medullary region. High densities of 15__R__‐[^3^H]TIC binding sites were shown in the dorsal part of the lateral septal nucleus, thalamic nuclei, limbic structures, and some of the cortical regions. Scatchard plot analysis showed two

Author

Yumiko Watanabe; Kiyoshi Matsumura; Hajime Takechi; Koichi Kato; Hiroshi Morii; Margareta Björkman; Bengt Langstrom; Ryoji Noyori; Masaaki Suzuki; Yasuyoshi Watanabe

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0022-3042)

Published

1999

Language

EN

Field

Neuroscience (Life Sciences)