Can I read Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose on EtoBox?

Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose by Mudinoor, Akshata R.; Goodwin, Peter M.; Rao, Raghavendra U.; Karuna, Nardrapee; Hitomi, Alex; Nill, Jennifer; Jeoh, Tina is a Engineering article available to read on EtoBox.

What is Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose about?

## Abstract ## Background Molecular-scale mechanisms of the enzymatic breakdown of cellulosic biomass into fermentable sugars are still poorly understood, with a need for independent measurements of enzyme kinetic parameters. We measured binding times of cellobiohydrolase Trichoderma reesei Cel7A (Cel7A) on celluloses using wild-type Cel7A (WT~intact~), the catalytically deficient mutant Cel7A E212Q (E212Q~intact~) and their proteolytically isolated catalytic domains (CD) (WT~core~ and E212Q~core~, respectively). The binding time distributions were obtained from time-resolved, super-resolution images of fluorescently labeled enzymes on cellulose obtained with total internal reflection fluorescence microscopy. ## Results Binding of WT~intact~ and E212Q~intact~ on the recalcitrant algal cellulose (AC) showed two bound populations: ~ 85% bound with shorter residence times of < 15 s while ~ 15% were effectively immobilized. The similarity between binding times of the WT and E212Q suggests that the single point mutation in the enzyme active site does not affect the thermodynamics of binding of this enzyme. The isolated catalytic domains, WT~core~ and E212Q~core~, exhibited three binding

Who reads Interfacial molecular interactions of cellobiohydrolase Cel7A and its variants on cellulose?

Author

Mudinoor, Akshata R.; Goodwin, Peter M.; Rao, Raghavendra U.; Karuna, Nardrapee; Hitomi, Alex; Nill, Jennifer; Jeoh, Tina

Publisher

BioMed Central; Springer (Biomed Central Ltd.); [London]: BioMed Central; Springer Science and Business Media LLC (ISSN 1754-6834)

Published

2020

Language

EN

ISBN

9781566994910

Field

Engineering (Physical Sciences)