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A Degradable Hyperbranched Poly(ester amine) Based on Poloxamer Diacrylate and Polyethylenimine as a Gene Carrier by Tae Hee Kim; Seung Eun Cook; Rohidas B. Arote; Myung‐Haing Cho; Jae Woon Nah; Yun Jaie Choi; Chong Su Cho is a Materials Science article available to read on EtoBox.
What is A Degradable Hyperbranched Poly(ester amine) Based on Poloxamer Diacrylate and Polyethylenimine as a Gene Carrier about?
## Abstract Polyethylenimine (PEI) is a well‐known cationic polymer which has high transfection efficiency due to its buffering effect. However, nondegradability, cytotoxicity, aggregation, and short‐circulation time __in vivo__ still need to be overcome for a successful gene delivery. Degradable, hyperbranched poly(ester amine)s (PEAs) based on poloxamer diacrylate and low molecular weight branched PEI, were successfully synthesized and evaluated as a nonviral gene carrier. The PEAs were obtained in significant yields through Michael type addition reaction of diacrylate monomers and low molecular weight branched PEI. Analysis of degradation products by the reduction in molecular weight demonstrated that PEAs degrade in a controlled fashion. The PEA showed good DNA binding ability and the sizes of complexes under physiological condition were below 150 nm, implicating its potential for intracellular delivery. It showed lower cytotoxicity in three different cell lines (A549, 293T, and HepG2) compared with PEI 25K. PEAs showed much higher transfection efficiencies in three cell lines compared with PEI 25K and PEI 1.8K, and revealed little serum dependency in A549 cell line when the co
Who reads A Degradable Hyperbranched Poly(ester amine) Based on Poloxamer Diacrylate and Polyethylenimine as a Gene Carrier?
It is typically read by researchers, students, and practitioners in Materials Science.
- Author
- Tae Hee Kim; Seung Eun Cook; Rohidas B. Arote; Myung‐Haing Cho; Jae Woon Nah; Yun Jaie Choi; Chong Su Cho
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1616-5187)
- Published
- 2007
- Language
- EN
- Field
- Materials Science (Physical Sciences)