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Promoter hypermethylation of MGMT is associated with protein loss in gastric carcinoma by Naohide Oue; Hideo Shigeishi; Hiroki Kuniyasu; Hiroshi Yokozaki; Kazuya Kuraoka; Reiko Ito; Wataru Yasui is a scholarly article available to read on EtoBox.

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Aberrant methylation of CpG islands within promoter regions is associated with transcriptional inactivation of various tumor suppressor genes in neoplasms. Recently, O 6 -methylguanine-DNA methyltransferase, MGMT, was shown to be hypermethylated in certain carcinomas, resulting in loss of MGMT protein. We studied DNA methylation of CpG islands of the MGMT gene by methylation specific PCR in 26 gastric carcinoma tissues and 8 gastric carcinoma cell lines for comparison with levels of MGMT protein expression. In addition, we examined p53 mutation status in the same tissues by PCR-SSCP analysis for comparison with MGMT protein expression levels. In total, promoter hypermethylation of the MGMT gene was found in 8 (31%) of the 26 gastric carcinomas with reduced expression of MGMT protein, whereas the hypermethylation was not detected in the 18 carcinomas with non-reduced MGMT expression. MGMT protein expression levels were associated with promoter hypermethylation of MGMT (p ‫؍‬ 0.0001; Mann-Whitney test); however, MGMT expression was not associated with p53 mutation status (p ‫؍‬ 0.461; Mann-Whitney test). Among in gastric carcinoma cell lines, the TMK-1 cell line showed loss of the MG

Author

Naohide Oue; Hideo Shigeishi; Hiroki Kuniyasu; Hiroshi Yokozaki; Kazuya Kuraoka; Reiko Ito; Wataru Yasui

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2001

Language

EN