Can I read Reverse Transcription ☆ on EtoBox?

Reverse Transcription ☆ by R.F. Schinazi; J.J. Kohler; B. Kim is a book available to read on EtoBox.

What is Reverse Transcription ☆ about?

Normal transcription is the process of synthesizing ribonucleic acid (RNA) from deoxynucleic acid (DNA). Briefly, genetically encoded sequences from the DNA genome are copied into RNA code, which is subsequently used as template and translated into specific protein(s). Commonly, in cells, a (DNA) gene that encodes a particular protein will be copied into "messenger RNA" (mRNA), and then the mRNA is transported to the section of the cell where proteins are made. In contrast, reverse transcription is the opposite process, that is, copying RNA into DNA. Single-stranded RNA is transcribed into single-stranded DNA by the RNAdependent DNA polymerase enzyme, reverse transcriptase (RT). This enzyme was independently discovered by Howard Temin and David Baltimore in 1970. Reverse transcription has a high error rate in transcribing RNA into DNA, since, unlike any other DNA polymerases, RT has no proofreading ability. This high error rate allows mutations to accumulate at an accelerated rate.Reverse transcription is commonly used in research in a technique called reverse transcription-polymerase chain reaction (RT-PCR). With the help of an RT, RNA can be transcribed into DNA, thus making PCR

Author

R.F. Schinazi; J.J. Kohler; B. Kim

Publisher

Elsevier

Published

2017

Language

EN