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Can I read Identification of amino acid phenylthiohydantoins by gradient, high-performance liquid chromatography on Spherisorb S5-ODS on EtoBox?
Identification of amino acid phenylthiohydantoins by gradient, high-performance liquid chromatography on Spherisorb S5-ODS by Peter W. Moser; Egon E. Rickli is a Chemistry article available to read on EtoBox.
What is Identification of amino acid phenylthiohydantoins by gradient, high-performance liquid chromatography on Spherisorb S5-ODS about?
A problem in the sequence determination of peptides arid proteins by the Edman method is that of the identification of the liberated amino acids which are present as their phenylthiohydantoin derivatives (PTH-amino acids). This task has usuaIly been soIved by applying techniques such as gas and/or thin-layer chromatography, hydrolysis to the free amino acids, etc. Over the past few years an increasing number of publications have described the introduction and application of highperformance liquid chromatography (HPLC) in this field. The advantage of HPLC lies in the direct identification of the PTH-amino acids without further derivatization and in their quantitative estimation which is possible with modern equipment. Current methods employ one-or two-column procedures with either gradient elution or isocratic deveIopment of the chromatogram'-". We now report a method which aliows the identification of the PTH-amino acids on a commerciaIIy avaiIabIe reversed-phase system in a single analysis with a gradient elution program.
Who reads Identification of amino acid phenylthiohydantoins by gradient, high-performance liquid chromatography on Spherisorb S5-ODS?
It is typically read by researchers, students, and practitioners in Chemistry.
- Author
- Peter W. Moser; Egon E. Rickli
- Publisher
- Elsevier Science; Elsevier ; Elsevier BV (ISSN 1873-3778)
- Published
- 1979
- Language
- EN
- Field
- Chemistry (Physical Sciences)