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Cross Priming Amplification Mechanism by mateus_laguardia is a document available to read on EtoBox.
1. Cross priming amplification (CPA) is an isothermal DNA amplification technique that uses multiple primers, including at least one cross primer, and a strand displacement DNA polymerase to exponentially amplify a target sequence without thermal cycling. 2. The basic mechanism of single crossing CPA involves a cross primer binding to the target and being displaced by a downstream primer, introducing a new priming site. This displaced strand then serves as a template for other primers, producing two differ
- Author
- mateus_laguardia
- Language
- EN