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Differential Effects of Frozen Storage on the Molecular Detection of Bacterial Taxa That Inhabit the Nasopharynx by Brenda A Kwambana; Nuredin I Mohammed; David Jeffries; Mike Barer; Richard A Adegbola; Martin Antonio is a Medicine article available to read on EtoBox.
What is Differential Effects of Frozen Storage on the Molecular Detection of Bacterial Taxa That Inhabit the Nasopharynx about?
## Background Frozen storage often precedes metagenomic analysis of biological samples; however, the freezing process can have adverse effects on microbial composition. The effect of freezing on the detection of bacteria inhabiting the infant nasopharynx, a major reservoir of bacterial pathogens, was investigated. ## Methods 16S ribosomal RNA (rRNA) gene-based terminal restriction fragment length polymorphism (T-RFLP) analysis of nasopharyngeal (NP) swabs from twelve Gambian infants was employed. NP swabs were analysed within hours of collection and then after 30 days of storage at -70°C. ## Results There was substantial heterogeneity among subjects with respect to the effect of freezing on the number of operational taxonomic units (OTUs) detected. Nevertheless, the mean number of OTUs decreased after frozen storage and the relative abundance for 72% of the OTUs changed by less than 0.5% after deep frozen storage. There were differences in the odds of detection and relative abundance of OTUs matched with __Moraxella sp__., __Haemophilus sp__./__Burkholderia sp.__, and __Pseudomonas sp__. A strong interaction between sex and the effect of freezing was found, whereby there was no sig
Who reads Differential Effects of Frozen Storage on the Molecular Detection of Bacterial Taxa That Inhabit the Nasopharynx?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Brenda A Kwambana; Nuredin I Mohammed; David Jeffries; Mike Barer; Richard A Adegbola; Martin Antonio
- Publisher
- BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC (ISSN 1472-6890)
- Published
- 2011
- Language
- EN
- ISBN
- 9781472689016
- Field
- Medicine (Health Sciences)