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Functional and kinetic characterization of granulocyte colony‐stimulating factor‐primed CD34^−^ human stem cells by Roberto M. Lemoli; Francesco Bertolini; Maria T. Petrucci; Chiara Gregorj; Maria R. Ricciardi; Miram Fogli; Antonio Curti; Cristina Rabascio; Simona Pandolfi; Sergio Ferrari; Robin Fo; Michele Baccarani; Agostino Tafuri is a Medicine article available to read on EtoBox.
What is Functional and kinetic characterization of granulocyte colony‐stimulating factor‐primed CD34^−^ human stem cells about?
**Summary.** We assessed the functional properties and the kinetic status __in vitro__, and the engraftment potential __in vivo__ of human haematopoietic stem cells according to the expression of CD34 antigen. Lin^−^CD34^−^ and Lin^−^CD34^+^ cells were isolated from granulocyte colony‐stimulating factor‐primed peripheral blood (PB) cells of healthy donors. The CD34^−^ cell fraction did not contain either clonogenic cells in semisolid culture or long‐term culture initiating cells (LTC‐IC). However, stroma‐dependent liquid cultures and cytokines induced CD34 expression on a minority of stem cells, acquisition of clonogenic capacity and generation of LTC‐IC. Significantly higher percentages of quiescent G~0~ cells and lower percentages of cycling G~1~ cells were found in Lin^−^CD34^−^ cells when compared with Lin^−^CD34^+^ cells. Kinetic quiescence of Lin^−^CD34^−^ cells was associated with a significantly higher expression of the negative regulators of the cell cycle, p27^Kip1^ and p21^cip1/waf1^. Cytokine‐mediated induction of CD34, __in vitro__, resulted in cycling of stem cells and downregulation of p27. There was a higher rate of human long‐term engraftment in immunocompromised n
Who reads Functional and kinetic characterization of granulocyte colony‐stimulating factor‐primed CD34^−^ human stem cells?
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- Author
- Roberto M. Lemoli; Francesco Bertolini; Maria T. Petrucci; Chiara Gregorj; Maria R. Ricciardi; Miram Fogli; Antonio Curti; Cristina Rabascio; Simona Pandolfi; Sergio Ferrari; Robin Fo; Michele Baccarani; Agostino Tafuri
- Publisher
- John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0007-1048)
- Published
- 2003
- Language
- EN
- Field
- Medicine (Health Sciences)