About this document
DNA Probes in Gel Electrophoresis Analysis by Neomita Carvalho is a document available to read on EtoBox.
The scientists used a radioactively labelled DNA probe to detect the presence of the SUT1 gene in tobacco plant leaves. They would isolate DNA from the leaves, break it into fragments through restriction enzyme digestion, separate the fragments by size using gel electrophoresis, and transfer them to a membrane. The probe would then bind to any fragments containing the SUT1 gene sequence, revealing its presence through radioactive detection. The scientists genetically modified some tobacco plants to produc
- Author
- Neomita Carvalho
- Language
- EN