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Knockdown of SMAD3 inhibits the growth and enhances the radiosensitivity of lung adenocarcinoma via p21 in vitro and in vivo by Niu, Hao; Huang, Yiwei; Yan, Li; Zhang, Li; Zhao, Mengnan; Lu, Tao; Yang, Xiaodong; Chen, Zhengcong; Zhan, Cheng; Shi, Yu; Wang, Qun is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Knockdown of SMAD3 inhibits the growth and enhances the radiosensitivity of lung adenocarcinoma via p21 in vitro and in vivo about?
Radiotherapy is an effective approach for the treatment of lung adenocarcinoma. However, evidence suggests that lung adenocarcinoma can easily develop tolerance to radiotherapy. The purpose of this study was to investigate the effect and mechanism of SMAD3 on the radiosensitivity of lung adenocarcinoma __in vitro__ and __in vivo__. We found that knockdown of SMAD3 using two short hairpin RNAs in lentivirus vectors significantly inhibited cell growth and increased radiosensitivity of the lung adenocarcinoma cell lines A549, H1299, and H1975. Using RNA sequencing and bioinformatics analyses, we found that the significantly differentially expressed genes in SMAD3 knockdown cells were mainly enriched in the cell cycle process. We then showed that knockdown of SMAD3 significantly reduced expression of cyclin-dependent kinase inhibitor 1 (p21) and increased the proportion of G2/M phase cells and the radiosensitivity of lung adenocarcinoma. Chromatin immunoprecipitation results in the Gene Expression Omnibus (GEO) database and our luciferase assay verified that SMAD3 directly bound the p21 promoter. A series of rescue experiments showed that overexpression of p21 partly reversed the effec
Who reads Knockdown of SMAD3 inhibits the growth and enhances the radiosensitivity of lung adenocarcinoma via p21 in vitro and in vivo?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Niu, Hao; Huang, Yiwei; Yan, Li; Zhang, Li; Zhao, Mengnan; Lu, Tao; Yang, Xiaodong; Chen, Zhengcong; Zhan, Cheng; Shi, Yu; Wang, Qun
- Publisher
- Ivyspring International Publisher; Bluehaven (N.S.W.): Ivyspring International, 2004- (ISSN 1449-2288)
- Published
- 2020
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)