Can I read Disulfide conformation and design at helix N-termini on EtoBox?

Disulfide conformation and design at helix N-termini by S. Indu; Senthil T. Kumar; Sudhir Thakurela; Mansi Gupta; Ramachandra M. Bhaskara; C. Ramakrishnan; Raghavan Varadarajan is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Disulfide conformation and design at helix N-termini about?

## Abstract To understand structural and thermodynamic features of disulfides within an α‐helix, a non‐redundant dataset comprising of 5025 polypeptide chains containing 2311 disulfides was examined. Thirty‐five examples were found of intrahelical disulfides involving a CXXC motif between the N‐Cap and third helical positions. GLY and PRO were the most common amino acids at positions 1 and 2, respectively. The N‐Cap residue for disulfide bonded CXXC motifs had average (φ,ψ) values of (−112 ± 25.2°, 106 ± 25.4°). To further explore conformational requirements for intrahelical disulfides, CYS pairs were introduced at positions N‐Cap‐3; 1,4; 7,10 in two helices of an __Escherichia coli__ thioredoxin mutant lacking its active site disulfide (nSS Trx). In both helices, disulfides formed spontaneously during purification only at positions N‐Cap‐3. Mutant stabilities were characterized by chemical denaturation studies (in both oxidized and reduced states) and differential scanning calorimetry (oxidized state only). All oxidized as well as reduced mutants were destabilized relative to nSS Trx. All mutants were redox active, but showed decreased activity relative to wild‐type thioredoxin. S

Who reads Disulfide conformation and design at helix N-termini?

Author

S. Indu; Senthil T. Kumar; Sudhir Thakurela; Mansi Gupta; Ramachandra M. Bhaskara; C. Ramakrishnan; Raghavan Varadarajan

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley-Liss Inc; Wiley (ISSN 0887-3585)

Published

2009

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)