Can I read Interactions between janus and bcd cortical pattern mutants in Tetrahymena thermophila: An investigation of intracellular patterning mechanisms using double-mutant analysis on EtoBox?

Interactions between janus and bcd cortical pattern mutants in Tetrahymena thermophila: An investigation of intracellular patterning mechanisms using double-mutant analysis by Eric Stephen Cole; Joseph Frankel; Leslie Meek Jenkins is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Interactions between janus and bcd cortical pattern mutants in Tetrahymena thermophila: An investigation of intracellular patterning mechanisms using double-mutant analysis about?

An analysis of bcd, janA; bcd, janB; and bcd, janC double-mutant phenotypes in Tetrahymena thermophila has allowed us to examine patterning processes affected by two different classes of mutations. bcd brings about a broadening of the oral and contractile vacuole pore domains in the ciliate cortex, while the janus mutations generate a mirror-image duplication of the ventral cortical pattern. We observed both bcd and janus characteristics expressed in the double mutants, as well as features unique to the double-mutant. Temperature-shift experiments employing the temperature-sensitive janB mutation in a double-mutant (bcd, janB) combination allowed us to observe the changes in pattern as a mirror-image geometry was brought into expression and subsequently removed within the bcd, janB double homozygote. These experiments suggest that there are multiple pattern-mechanisms at work with differing kinetics of expression in the ciliate cortex. We discuss how the bcd mutation could influance expression of the janus mutations in light of a model previously proposed to account for the janus phenotype.

Who reads Interactions between janus and bcd cortical pattern mutants in Tetrahymena thermophila: An investigation of intracellular patterning mechanisms using double-mutant analysis?

Author

Eric Stephen Cole; Joseph Frankel; Leslie Meek Jenkins

Publisher

Springer-Verlag; Springer Verlag; Springer Science and Business Media LLC (ISSN 1432-041X)

Published

1988

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)