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Western Blot Protocol and Reagents by Rafif Alvian is a document available to read on EtoBox.
What is Western Blot Protocol and Reagents about?
This document provides instructions for performing a western blot. It lists the required reagents which include transfer buffer, TBST, powdered milk solution, primary and secondary antibodies, and Pierce Super Signal. It also lists the required equipment which includes a nitrocellulose membrane, filter paper, X-cell blot module, western blot sponges, power pack, and Fuji camera. It then provides a step-by-step method for performing a western blot on a single membrane, including transferring the gel to the m
- Author
- Rafif Alvian
- Language
- EN