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Ovarian tumor cell detection in peripheral blood progenitor cells harvests by RT‐PCR by Hitoshi Kurata; Koich Takakuwa; Ikunosuke Tsuneki; Yoichi Aoki; Kenichi Tanaka is a Medicine article available to read on EtoBox.

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**Background.** To evaluate the frequency of tumor cell contamination in autologous peripheral‐blood progenitor cells from patients with ovarian cancer, and to determine the impact of infusing such cells on relapses after high‐dose chemotherapy. **Methods.** Seventy‐three samples of peripheral‐blood progenitor cells from 24 ovarian cancer patients were studied for contaminated tumor cells by cytokeratin 7 and cytokeratin 20 reverse transcriptase polymerase chain reaction. **Results.** Tumor cell contamination in peripheral‐blood progenitor cells was detected in 11 of 24 patients (46%) and, among these, in four of 11 patients who received transplantations of peripheral‐blood progenitor cells. There was no trend towards longer relapse‐free survival in patients infused with cytokeratin‐negative peripheral‐blood progenitor cells as compared with positive ones. Interestingly, two of four patients who received transplantations of peripheral‐blood progenitor cells containing tumor cells were free from progression at 20 and 41 months after transplantation. **Conclusion.** Tumor cell contamination of peripheral‐blood progenitor cells was frequently noted by transcriptase polymerase chain re

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Author

Hitoshi Kurata; Koich Takakuwa; Ikunosuke Tsuneki; Yoichi Aoki; Kenichi Tanaka

Publisher

Informa plc; Wiley (Blackwell Publishing); Wiley-Blackwell; Wiley (ISSN 0001-6349)

Published

2002

Language

EN

Field

Medicine (Health Sciences)