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Comparison of a novel ColiPlateTM kit and the standard membrane filter technique for enumerating total coliforms and Escherichia coli bacteria in water by Ran Lifshitz; Renu Joshi is a Environmental Science article available to read on EtoBox.
What is Comparison of a novel ColiPlateTM kit and the standard membrane filter technique for enumerating total coliforms and Escherichia coli bacteria in water about?
The new ColiPlate CP kit was evaluated comparatively with the standard membrane filter ( ) MF technique for enumerating total coliforms and Escherichia coli in water. In testing natural water ( 2 ) samples, good correlations were observed for enumerating total coliforms R = 0.84 and E. coli ( 2 ) R = 0.95 . However, counts of E. coli population density estimated by CP were 47% higher than counts estimated by MF. With the water samples spiked with culture-grown E. coli cells, the correlation ( 2 ) ( 2 ) between the methods was strong for both total coliforms R = 0.95 and for E. coli R = 0.94 . E. coli densities were estimated to be 20% higher using CP compared with MF. Samples spiked with ( ) rehydrated freeze-dried E. coli cells with high portion of injured or weakened cells showed a strong ( 2 ) correlation between the two methods R = 0.93, for either total coliforms or E. coli . However, estimated ( ) total coliforms and E. coli densities were higher by CP than MF counts 38 and 168%, respectively . The CP test is therefore considered a more reliable method than the traditional MF for enumerating E. coli in samples with high levels of injured or weakened cells.
Who reads Comparison of a novel ColiPlateTM kit and the standard membrane filter technique for enumerating total coliforms and Escherichia coli bacteria in water?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Ran Lifshitz; Renu Joshi
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1053-4725)
- Published
- 1998
- Language
- EN
- Field
- Environmental Science (Physical Sciences)