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Nicotine affects the signaling of the death pathway, reducing the response of head and neck cancer cell lines to DNA damaging agents by Naoyoshi Onoda; Alisar Nehmi; David Weiner; Sunita Mujumdar; Randolph Christen; Gerrit Los is a Medicine article available to read on EtoBox.

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## Abstract ## Background Growing evidence suggests that tobacco can affect the responsiveness of cancer cells to treatment, particularly those of head and neck cancer. This article describes the effects of nicotine on the signaling of the death pathway, resulting in a decreased cytotoxicity of various anticancer agents such as cisplatin and gamma‐radiation. ## Methods Colony‐forming assays (CFA), using the head and neck cancer cell lines UMSCC10b and UMSCC5 and DNA fragmentation assays, were used to determine the effect of nicotine on cytotoxicity of various anticancer agents, whereas PCR and a JNK activity test were used to study the effect of nicotine on message expression levels and activity of the JNK signaling pathway. ## Results Nicotine consistently reduced the cytotoxic effect of DNA‐damaging agents, such as cisplatin, UV, and gamma radiation, in UMSCC10b cells, increasing their IC~50~ values by twofold, 1.7‐fold, and 1.8‐fold, respectively. These results were confirmed in a second squamous cell carcinoma cell line (UMSCC5), demonstrating an increase in IC~50~ values for cDDP by twofold and 1.9‐fold in the UMSCC10b andUMSCC5, respectively. In addition, nicotine reduced the

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Author

Naoyoshi Onoda; Alisar Nehmi; David Weiner; Sunita Mujumdar; Randolph Christen; Gerrit Los

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 1043-3074)

Published

2001

Language

EN

Field

Medicine (Health Sciences)