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This document describes a simple method for detecting live Borrelia spirochaetes in human blood using classical microscopy techniques. The method involves taking a peripheral blood sample from patients suspected of having chronic borreliosis and examining slides of the blood under phase-contrast and fluorescence microscopy. Over 24-48 hours at room temperature, the low oxygen conditions between the slide and cover glass are hypothesized to cause Borrelia stages to become visible as they replicate. Various m

Author

abap2814

Language

EN