Can I read Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries on EtoBox?

Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries by Wilfried H. B. M. Levering; Wessel N. van Wieringen; Jaco Kraan; Wil A. M. van Beers; Kees Sintnicolaas; Dick J. van Rhenen; Jan W. Gratama is a Medicine article available to read on EtoBox.

What is Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries about?

## Abstract A biannual external quality assessment (EQA) scheme for flow cytometric lymphocyte immunophenotyping is operational in the Benelux countries since 1996. We studied the effects of the methods used on assay outcome, and whether or not this EQA exercise was effective in reducing between‐laboratory variation. Eighty test samples were distributed in 20 biannual send‐outs. Per send‐out, 50–71 participants were requested to enumerate CD3^+^, CD4^+^, and CD8^+^ T cells, B cells, and NK cells, and to provide methodological details. Participants received written debriefings with personalized recommendations after each send‐out. For this report, data were analyzed using robust multivariate regression. Five variables were associated with significant positive or negative bias of absolute lymphocyte subset counts: (i) platform methodology (i.e., single‐platform assays yielded lower CD4^+^ and CD8^+^ T‐cell counts than did dual‐platform assays); (ii) sample preparation technique (i.e., assays based on mononuclear cells isolation yielded lower T‐cell counts than those based on red cell lysis); (iii) gating strategies based on CD45 and sideward scatter gating of lymphocytes yielded high

Who reads Flow cytometric lymphocyte subset enumeration: 10 years of external quality assessment in the Benelux countries?

Author

Wilfried H. B. M. Levering; Wessel N. van Wieringen; Jaco Kraan; Wil A. M. van Beers; Kees Sintnicolaas; Dick J. van Rhenen; Jan W. Gratama

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Ltd.; Wiley (ISSN 1552-4949)

Published

2007

Language

EN

Field

Medicine (Health Sciences)