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Photoautotrophic nutrient utilization and limitation during semi‐continuous growth of Synechocystis sp. PCC6803 by Hyun Woo Kim; Raveender Vannela; Chao Zhou; Chris Harto; Bruce E. Rittmann is a scholarly article available to read on EtoBox.

What is Photoautotrophic nutrient utilization and limitation during semi‐continuous growth of Synechocystis sp. PCC6803 about?

Microbial photosynthesis presents a valuable opportunity to capture abundant light energy to produce renewable bioenergy and biomaterials. To understand the factors that control the productivity of photosynthetic microorganisms, we conducted a series of semi-continuous experiments using bench-scale photobioreactor (PBR) systems, the cyanobacterium Synechocystis PCC6803 (PCC6803), and light conditions imitating actual day-night light irradiance (LI). Our results demonstrate that using normal BG-11 medium resulted in severe phosphate (P i ) limitation for continuous operation. Mitigation of P i -limitation, by augmenting the P i content of BG-11, allowed higher biomass productivity; however, once P i -limitation was alleviated, limitation by inorganic carbon (C i ) or LI occurred. C i -limitation was detected by a low total C i concentration (<5 mg C/L) and high and fluctuating pH. C i -limitation was relieved by delivering more CO 2 , which led to a stable pH in the range of 7-9 and at least 5 mg/L of C i in HCO À 3 . LI limitation, evidenced by an average LI <14 W/m 2 for PCC6803, was induced by a high biomass concentration of 1,300 mg/L. Thus, this work provides quantitative tools

Author

Hyun Woo Kim; Raveender Vannela; Chao Zhou; Chris Harto; Bruce E. Rittmann

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); Wiley - V C H Verlag GmbbH & Co.; Wiley (ISSN 0006-3592)

Published

2010

Language

EN