Can I read Cross-reactivity of an antineurofilament antibody with a troponin-T isoform on EtoBox?

Cross-reactivity of an antineurofilament antibody with a troponin-T isoform by Dr. Michito Takagi; Dr. Hiromi Takano-Ohmuro; Dr. Toshitaka Nakamura; Dr. Mr. Hajime Kawahara; Dr. Teruo Shimizu; Dr. Takashi Obinata; Dr. Kazuhiro Kohama is a Medicine article available to read on EtoBox.

What is Cross-reactivity of an antineurofilament antibody with a troponin-T isoform about?

Histochemical examination has revealed that a monoclonal antibody raised against the 200-kilodalton (kd) subunit of neurofilament reacts not only with neural tissue but with type 1 and type 2C fibers of skeletal muscle. To identify the cross-reactive substance(s), rat soleus muscle, which includes type I and type 2C fibers, was separated into a soluble fraction and an insoluble myofibrillar fraction. Western blot analysis demonstrated that the antibody reacted with 30-kd and 36-kd polypeptides in the soluble fraction and with a 38-kd polypeptide in the insoluble fraction. We isolated a fraction that contained the 38-kd polypeptide from the insoluble fraction. The material in this fraction bound to F-actin. Two-dimensional electrophoresis of the fraction showed that the 38-kd polypeptide was part of the group of isoforms of troponin-T, which was identified by a monoclonal antibody developed against troponin-T. Thus, we conclude that this antineurofilament antibody recognizes one of the isoforms of troponin-T in type 1 and type 2C fibers.

Who reads Cross-reactivity of an antineurofilament antibody with a troponin-T isoform?

Author

Dr. Michito Takagi; Dr. Hiromi Takano-Ohmuro; Dr. Toshitaka Nakamura; Dr. Mr. Hajime Kawahara; Dr. Teruo Shimizu; Dr. Takashi Obinata; Dr. Kazuhiro Kohama

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0148-639X)

Published

1989

Language

EN

Field

Medicine (Health Sciences)