Opening book details…
Can I read Immunohistochemical detection of P-glycoprotein in human tumor cells with a low degree of drug resistance on EtoBox?
Immunohistochemical detection of P-glycoprotein in human tumor cells with a low degree of drug resistance by Henricus J. Broxterman; Herbert M. Pinedo; Catharina M. Kuiper; Jacobus J. M. Van Der Hoeven; Pieter De Lange; Jasper J. Quak; Rik J. Scheper; Hiskias G. Keizer; Gerrit Jan Schuurhus; Jan Lankelma is a Medicine article available to read on EtoBox.
What is Immunohistochemical detection of P-glycoprotein in human tumor cells with a low degree of drug resistance about?
We report the immunohistochemical detection of the 170-180 kDa multi-drug-resistance-related Pglycoprotein in human tumor cells with a low level of resistance. A series of human squamous lung cancer cell lines with increasing levels of resistance to doxorubicin (DOX) was developed and stained for P-glycoprotein, using the JSB-I MAb. Subline SW 1573150A with a 4to 6-fold cross-resistance to daunorubicin (DNR) and vincristine (VCR) showed rather uniform positive staining for P-glycoprotein apparently at cytoplasmic sites. Only in cells with higher degrees of resistance (> 10-fold) could plasmamembrane-associated P-glycoprotein be made visible. DNR efflux was increased in SW1573150A as compared to the parent line SW I573 (52 and 70% DNR were retained during 3 min efflux respectively). Verapamil partially reversed DNR and VCR resistance in SW1573150A. Cells obtained from a metastasized renal cell carcinoma and cultured in vitro stained in a similar way to SW l573/50A and showed some sensitivity to verapamil modulation of VCR cytotoxicity. Our results suggest that weakly resistant cancer cells obtained from patients can be routinely detected with JSB-I on cytospins, and implicate that i
Who reads Immunohistochemical detection of P-glycoprotein in human tumor cells with a low degree of drug resistance?
It is typically read by researchers, students, and practitioners in Medicine.
- Author
- Henricus J. Broxterman; Herbert M. Pinedo; Catharina M. Kuiper; Jacobus J. M. Van Der Hoeven; Pieter De Lange; Jasper J. Quak; Rik J. Scheper; Hiskias G. Keizer; Gerrit Jan Schuurhus; Jan Lankelma
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)
- Published
- 1989
- Language
- EN
- Field
- Medicine (Health Sciences)