Can I read Raman spectroscopy of supercoiled and nicked ColE1 plasmid on EtoBox?

Raman spectroscopy of supercoiled and nicked ColE1 plasmid by William A. Christens-Barry; James C. Martin; Jacob Lebowitz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Raman spectroscopy of supercoiled and nicked ColE1 plasmid about?

Native supercoiled and nicked ColEl DNA were examined using laser Raman spectroscopy. ColEl contains 6646 base pairs (bp) and, when supercoiled, approximately 47 negative supercoils. An analytical buoyant density gradient centrifugation technique developed by Burke and Bauer was scaled to preparative quantities, and used to isolate the supercoiled plasmid fraction from its nicked counterpart. This procedure allowed enriched fractions of the supercoiled plasmid to be extracted without the use of the optical contaminant ethidium bromide. The intensities of several Raman bands were altered between the spectra of the two topological forms. Notably absent were any changes in bands arising from cytosine and guanine vibrations. The observed changes are interpreted in terms of the polymorphic structures which have been observed in many DNA structural studies. The results of this study suggest that accommodation of supercoiling takes place chiefly in A-T base pairs and backbone moieties, without substantial modification of G-C base-pair structure. Premelting effects may account for the observed changes, including a slight shift to lower frequency of a band known to be responsive to base-pai

Who reads Raman spectroscopy of supercoiled and nicked ColE1 plasmid?

Author

William A. Christens-Barry; James C. Martin; Jacob Lebowitz

Publisher

Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0006-3525)

Published

1989

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)