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Synthesis and proof‐of‐function of a [^14^C]‐labelled form of the plant iron chelator nicotianamine using recombinant nicotianamine synthase from barley by Lars Schmiedeberg; Claudia Krüger; Udo W. Stephan; Helmut Bäumlein; Rüdiger Hell is a Agricultural and Biological Sciences article available to read on EtoBox.

What is Synthesis and proof‐of‐function of a [^14^C]‐labelled form of the plant iron chelator nicotianamine using recombinant nicotianamine synthase from barley about?

**The amino acid nicotianamine (NA) is essential for micronutrient metabolism in plants. Lack of NA results in a chlorotic phenotype and oxidative stress, since NA is a chelator of iron and other metal nutrients. To investigate the precise cellular function of NA in micronutrient transport and homeostasis, a protocol for the production of [^14^C]‐labelled NA was developed. Recombinant NA synthase was used to generate [^14^C]‐NA from [^14^C]‐__S__‐adenosylmethionine. After purification by solid‐phase ion exchange about 66% yield was achieved. The identity of the [^14^C]‐NA with chemically synthesized NA was demonstrated by several independent methods, including two TLC systems, two HPLC systems and immuno‐detection. Moreover, biological function was shown by complementation of the __Lycopersicon esculentum__ mutant __chloronerva__ that is free of NA due to a defect in NA synthase. Proof‐of‐function for the produced [^14^C]‐NA as a suitable tool for transport studies was provided monitoring the distribution of [^14^C]‐NA after feeding to tomato and __Ricinus communis__ seedlings.**

Who reads Synthesis and proof‐of‐function of a [^14^C]‐labelled form of the plant iron chelator nicotianamine using recombinant nicotianamine synthase from barley?

Author

Lars Schmiedeberg; Claudia Krüger; Udo W. Stephan; Helmut Bäumlein; Rüdiger Hell

Publisher

John Wiley and Sons; Wiley (Blackwell Publishing); Blackwell Publishing Inc.; Wiley (ISSN 0031-9317)

Published

2003

Language

EN

Field

Agricultural and Biological Sciences (Life Sciences)