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Cancer/testis antigen expression and specific cytotoxic T lymphocyte responses in non small cell lung cancer by Célia Groeper; Franco Gambazzi; Paul Zajac; Lukas Bubendorf; Michel Adamina; Rachel Rosenthal; Hans-Reinhard Zerkowski; Michael Heberer; Giulio C. Spagnoli is a Medicine article available to read on EtoBox.

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## Abstract Non small cell lung cancers (NSCLC) express cancer/testis antigens (CTA) genes and MAGE‐A expression correlates with poor prognosis in squamous cell carcinomas. We addressed cytotoxic T lymphocytes (CTL) responses to HLA class I restricted CTA epitopes in TIL from NSCLC in an unselected group of 33 patients consecutively undergoing surgery. Expression of MAGE‐A1, ‐A2, ‐A3, ‐A4, ‐A10, ‐A12 and NY‐ESO‐1 CTA genes was tested by quantitative RT‐PCR. Monoclonal antibodies (MAb) recognizing MAGE‐A and NY‐ESO‐1 CTA were used to detect CTA by immunohistochemistry. CD8^+^ TIL obtained from tumors upon culture with anti CD3 and anti CD28 mAb and IL‐2 were stimulated with autologous mature DC (mDC) and HLA‐A\*0101 restricted MAGE‐A1~161–169~ or MAGE‐A3~168–176~ peptides or HLA‐A\*0201 restricted MAGE‐A4~230–239~, MAGE‐A10~254–262~, NY‐ESO‐1~157–165~ or multi‐MAGE‐A (YLEYRQVPV) peptides or a recombinant vaccinia virus (rVV) encoding MAGE‐A and NY‐ESO‐1 HLA‐A\*0201 restricted epitopes and CD80 co‐stimulatory molecule. Specificity was assessed by ^51^Cr release and multimer staining. At least one CTA gene was expressed in tumors from 15/33 patients. In 10 specimens, at least 4 CTA ge

Who reads Cancer/testis antigen expression and specific cytotoxic T lymphocyte responses in non small cell lung cancer?

Author

Célia Groeper; Franco Gambazzi; Paul Zajac; Lukas Bubendorf; Michel Adamina; Rachel Rosenthal; Hans-Reinhard Zerkowski; Michael Heberer; Giulio C. Spagnoli

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square; Test accounts (ISSN 0020-7136)

Published

2006

Language

EN

Field

Medicine (Health Sciences)