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Preliminary association analysis of TLR9 gene polymorphisms and immune parameters in an Italian Holstein calves population by Elisabetta Catalani; Alessandro Nardone; Antonino Barone; Maria Teresa Scicluna; Gian Luca Autorino; Andrea Caprioli; Nicola Lacetera is a scholarly article available to read on EtoBox.

What is Preliminary association analysis of TLR9 gene polymorphisms and immune parameters in an Italian Holstein calves population about?

## Background This preliminary study was aimed at evaluating the association between single nucleotide polymorphisms (SNPs) on Toll like receptor 9 (TLR9) gene and some immunological parameters in a population of Italian Holstein calves. ## Methods The study was carried out in a commercial farm on 68 Holstein calves aging about 6 months. Genomic DNA was extracted from peripheral blood mononuclear cells (PBMC) and genotyped for nine SNPs on TLR9. Immunological parameters considered were the immunoglobulin (Ig) G titers against bovine herpesvirus 1, and the proliferative response of peripheral blood mononuclear cells to mitogens. For the association study, only results relative to the SNP located in the promoter region have been discussed. ## Results Among the nine SNPs expected, only eight were detected. Considering the SNP located in the promoter region, all three possible genotypes were observed, and their distribution was as follows: genotype a (n=34), b (n=19), and c (n=8). On the basis of their response to vaccine, calves were categorized as low (L, n=8), medium (M, n=45) and high responders (H, n=8). Although no significant association was found between genotypes and L, M or H

Author

Elisabetta Catalani; Alessandro Nardone; Antonino Barone; Maria Teresa Scicluna; Gian Luca Autorino; Andrea Caprioli; Nicola Lacetera

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2007-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1753-6561)

Published

2011

Language

EN