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Isolation and characterization of a novel lytic bacteriophage vB Efm LG62 infecting Enterococcus faecium by Qianyu Qu; Tao Chen; Penggang He; Huaixin Geng; Peibin Zeng; Guangxin Luan is a Medicine article available to read on EtoBox.

What is Isolation and characterization of a novel lytic bacteriophage vB Efm LG62 infecting Enterococcus faecium about?

Enterococcus faecium has been classified as a "high priority" pathogen by the World Health Organization. Enterococcus faecium has rapidly evolved as a global nosocomial pathogen with adaptation to the nosocomial environment and the accumulation of resistance to multiple antibiotics. Phage therapy is considered a promising strategy against difficult-totreat infections and antimicrobial resistance. In this study, we isolated and characterized a novel virulent bacteriophage, vB\_Efm\_LG62, that specifically infects multidrug-resistant E. faecium. Morphological observations suggested that the phage has siphovirus morphology, with an optimal multiplicity of infection of 0.001. One-step growth tests revealed that its latent growth was at 20 min, with a burst size of 101 PFU/cell. Phage vB\_Efm\_LG62 was verified to have a double-stranded genome of 42,236 bp (35.21% GC content), containing 66 predicted coding sequences as determined by whole genomic sequencing. No genes were predicted to have functions associated with virulence factors or antibiotic resistance, indicating that the phage vB\_Efm\_LG62 has good therapeutic potential. Our isolation and characterization of this highly efficie

Who reads Isolation and characterization of a novel lytic bacteriophage vB Efm LG62 infecting Enterococcus faecium?

Author

Qianyu Qu; Tao Chen; Penggang He; Huaixin Geng; Peibin Zeng; Guangxin Luan

Publisher

Springer Science and Business Media LLC

Published

2023

Language

EN

Field

Medicine (Life Sciences)