Can I read Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori on EtoBox?

Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori by Jinyong Zhang; Xiaoli Zhang; Xuhu Mao; Quanming Zou; Defeng Li is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori about?

Octaprenyl pyrophosphate synthase (OPPs) is involved in the synthesis of the side chains of ubiquinone and menaquinone and catalyzes consecutive condensation reactions of farnesyl pyrophosphate with isopentenyl pyrophosphate to generate polyprenyl pyrophosphate and pyrophosphate. In order to investigate the roles played by OPPs in the metabolism of ubiquinone and menaquinone and the enzymatic mechanisms of these enzymes, analysis of the structure-function relationship of OPPs from Helicobacter pylori was initiated. The gene for OPPs was cloned, the protein was expressed, purified and crystallized and a diffraction data set was collected to 2.00 Å resolution. The crystals belonged to space group P4(1)2(1)2 or P4(3)2(1)2, with unit-cell parameters a = b = 109.33, c = 103.41 Å.

Who reads Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori?

Author

Jinyong Zhang; Xiaoli Zhang; Xuhu Mao; Quanming Zou; Defeng Li

Publisher

John Wiley and Sons; International Union of Crystallography; John Wiley and Sons Ltd; International Union of Crystallography (IUCr) (ISSN 1744-3091)

Published

2011

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)