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What is Cell Culture about?
This document outlines a detailed protocol for cell preparation and gene delivery in HEK293T and C2C12 cells, focusing on plasmid transfection and adenoviral infection for gene overexpression studies, using MG53 as an example. It includes step-by-step instructions for cell culture, transfection, and infection, as well as troubleshooting tips for common issues. The protocol aims to facilitate efficient gene expression for downstream functional analysis in vitro.
- Author
- sayandeepbera2
- Language
- EN