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Detection of Microsatellite Instability in Cancers by Arbitrarily Primed-PCR Fingerprinting Using a Fluorescently Labeled Primer (FAP-PCR) by Yasuda, Jun; Kashiwabara, Hidefumi; Kawakami, Keita; Uematsu, Kazutsugu; Sugano, Kokichi; Perucho, Manuel; Sekiya, Takao is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

What is Detection of Microsatellite Instability in Cancers by Arbitrarily Primed-PCR Fingerprinting Using a Fluorescently Labeled Primer (FAP-PCR) about?

The microsatellite mutator phenotype (MMP), detected as a change in the number of repeating units in hundreds of thousands of microsatellite sequences in the tumor cell genome, underlies the carcinogenesis of a variety of tumors including sporadic and hereditary nonpolyposis colon cancers. This enhanced microsatellite instability was discovered using arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting of DMA from colon cancers. In this study, we found an arbitrary primer that can amplify multiple DMA fragments containing repeated sequences, including the poly A tracts found in the/Wu repeats of the human genome. The combined use of primer labeling with fluorescence and an automated DMA sequencing analysis of AP-PCR products (FAP-PCR) detected alterations in fingerprint bands in all DNA samples previously determined to belong to the MMP. Fluorescent AP-PCR fingerprinting using this single arbitrary primer provides a convenient and efficient method for detecting tumor specific fingerprint alterations that are usually undetectable by conventional fingerprinting.

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Author

Yasuda, Jun; Kashiwabara, Hidefumi; Kawakami, Keita; Uematsu, Kazutsugu; Sugano, Kokichi; Perucho, Manuel; Sekiya, Takao

Publisher

Walter de Gruyter GmbH (ISSN 0177-3593)

Published

1996

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)