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Fabrication and Characterization of Contact Lenses Bearing Surface-immobilized Layers of Intact Liposomes by Anne Danion; Heïdi Brochu; Yves Martin; Patrick Vermette is a Engineering article available to read on EtoBox.

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## Abstract Intact liposomes were immobilized onto soft contact lenses. In the first step, polyethylenimine was covalently bounded onto the hydroxyl groups available on the surface of a commercial contact lens (Hioxifilcon B). Then, NHS‐PEG‐biotin molecules were bounded onto the surface amine groups by carbodiimide chemistry. NeutrAvidin were bounded onto the PEG‐biotin layer. Liposomes containing PEG‐biotinylated lipids were docked onto the surface‐immobilized NeutrAvidin. Consecutive addition of further NeutrAvidin and liposome layers enabled fabrication of multilayers. Multilayers of liposomes were also produced by exposing contact lenses coated with NeutrAvidin to liposome aggregates produced by the addition of free biotin in solution. XPS revealed the immobilization of the different layers. By blocking with excess biotin surface‐immobilized NeutrAvidin on contact lenses bearing PEG‐biotin layers produced under cloud point conditions, ELISA showed that the docking of NeutrAvidin was dependent on biotin–NeutrAvidin affinity binding, with little evidence for nonspecific physisorption; however, it was not possible to differentiate specific versus nonspecific binding of NeutrAvidin

Who reads Fabrication and Characterization of Contact Lenses Bearing Surface-immobilized Layers of Intact Liposomes?

Author

Anne Danion; Heïdi Brochu; Yves Martin; Patrick Vermette

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1549-3296)

Published

2007

Language

EN

Field

Engineering (Physical Sciences)