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Can I read Fluoride disrupts intestinal epithelial tight junction integrity through intracellular calcium-mediated RhoA/ROCK signaling and myosin light chain kinase on EtoBox?
Fluoride disrupts intestinal epithelial tight junction integrity through intracellular calcium-mediated RhoA/ROCK signaling and myosin light chain kinase by Lianxin Li; Jinge Xin; Hesong Wang; Yadong Wang; Weiqi Peng; Ning Sun; Haonan Huang; Yanxi Zhou; Xingmei Liu; Yu Lin; Jing Fang; Bo Jing; Kangcheng Pan; Yan Zeng; Dong Zeng; Xiang Qin; Yang Bai; Xueqin Ni is a Environmental Science article available to read on EtoBox.
What is Fluoride disrupts intestinal epithelial tight junction integrity through intracellular calcium-mediated RhoA/ROCK signaling and myosin light chain kinase about?
Fluoride is a common contaminant of groundwater and agricultural commodity, which poses challenges to animal and human health. A wealth of research has demonstrated its detrimental effects on intestinal mucosal integrity; however, the underlying mechanisms remain obscure. This study aimed to investigate the role of the cytoskeleton in fluoride-induced barrier dysfunction. After sodium fluoride (NaF) treatment of the cultured Caco-2 cells, both cytotoxicity and cytomorphological changes (internal vacuoles or massive ablation) were observed. NaF lowered transepithelial electrical resistance (TEER) and enhanced paracellular permeation of fluorescein isothiocyanate dextran 4 (FD-4), indicating Caco-2 monolayers hyperpermeability. In the meantime, NaF treatment altered both the expression and distribution of the tight junction protein ZO-1. Fluoride exposure increased myosin light chain II (MLC2) phosphorylation and triggered actin filament (F-actin) remodeling. While inhibition of myosin II by Blebbistatin blocked NaF-induced barrier failure and ZO-1 discontinuity, the corresponding agonist Ionomycin had effects comparable to those of fluoride, suggesting that MLC2 serves as an effecto
Who reads Fluoride disrupts intestinal epithelial tight junction integrity through intracellular calcium-mediated RhoA/ROCK signaling and myosin light chain kinase?
It is typically read by researchers, students, and practitioners in Environmental Science.
- Author
- Lianxin Li; Jinge Xin; Hesong Wang; Yadong Wang; Weiqi Peng; Ning Sun; Haonan Huang; Yanxi Zhou; Xingmei Liu; Yu Lin; Jing Fang; Bo Jing; Kangcheng Pan; Yan Zeng; Dong Zeng; Xiang Qin; Yang Bai; Xueqin Ni
- Publisher
- Elsevier BV
- Published
- 2023
- Language
- EN
- Field
- Environmental Science (Physical Sciences)