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Embryonic vascular endothelial cells are malleable to reprogramming via Prox1 to a lymphatic gene signature by Harold Kim; Vicky PKH Nguyen; Tatiana V Petrova; Maribelle Cruz; Kari Alitalo; Daniel J Dumont is a scholarly article available to read on EtoBox.

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## Abstract ## Background __In vivo__ studies demonstrate that the Prox1 transcription factor plays a critical role in the development of the early lymphatic system. Upon Prox1 expression, early lymphatic endothelial cells differentiate from the cardinal vein and begin to express lymphatic markers such as VEGFR-3, LYVE-1 and Podoplanin. Subsequent __in vitro__ studies have found that differentiated vascular endothelial cells can be reprogrammed by Prox1 to express a lymphatic gene profile, suggesting that Prox1 can initiate the expression of a unique gene signature during lymphangiogenesis. While the __in vitro__ data suggest that gene reprogramming occurs upon Prox1 expression, it is not clear if this is a direct result of Prox1 in vascular endothelial cells __in vivo__. ## Results Overexpression of Prox1 in vascular endothelial cells during embryonic development results in the reprogramming of genes to that of a more lymphatic signature. Consequent to this overexpression, embryos suffer from gross edema that results in embryonic lethality at E13.5. Furthermore, hemorrhaging and anemia is apparent along with clear defects in lymph sac development. Alterations in junctional protein

Author

Harold Kim; Vicky PKH Nguyen; Tatiana V Petrova; Maribelle Cruz; Kari Alitalo; Daniel J Dumont

Publisher

BioMed Central; Springer (Biomed Central Ltd.); London: BioMed Central, 2001-; Springer Science and Business Media LLC; Society for Mining, Metallurgy and Exploration Inc. (ISSN 1471-213X)

Published

2010

Language

EN