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Isolation of prostate cancer-specific circulating tumor cells for androgen receptor splice variant 7 analysis: The AdnaTest ProstateCancer versus the Gilupi CellCollector by K. Ternov Kvorning; S.G. Jensen; P.B. Østergren; I. Balslev; M. Fode; H. Lindberg; C. Kistorp; R. Bisbjerg; G. Palapattu; J. Sønksen; E. Høgdall is a scholarly article available to read on EtoBox.

What is Isolation of prostate cancer-specific circulating tumor cells for androgen receptor splice variant 7 analysis: The AdnaTest ProstateCancer versus the Gilupi CellCollector about?

Introduction & Objectives: Circulating tumor cells (CTCs) have been widely studied as novel prognostic and predictive biomarkers in metastatic castration resistant prostate cancer (mCRPC). However, the process of CTC isolation and downstream reliable marker profiling of sparse amounts of CTC specific copies remains challenging. Several new commercially available methods have emerged to facilitate this process. The aim of this study was to evaluate the ability to isolate CTCs using the AdnaTest ProstateCancer (QIAGEN GmbH, Hilden, Germany) as compared to the Gilupi CellCollector (GILUPI GmbH, Potsdam, Germany). ## Materials & Methods: From July 2017 to May 2018 peripheral blood and CellCollector samples were prospectively collected from 28 men with high volume mCRPC (≥4 bone metastases with at least 1 beyond the vertebral bodies and pelvis) before commencing 1 st line mCRPC treatment. No patients had visceral metastases. 2 commercially available methods were compared: AdnaTest versus CellCollector. The AdnaTest used relies on positive selection of CTCs from blood samples by means of anti-EpCam and anti-HER2 antibodies. 18 ml of blood were collected from each patient for this analysi

Author

K. Ternov Kvorning; S.G. Jensen; P.B. Østergren; I. Balslev; M. Fode; H. Lindberg; C. Kistorp; R. Bisbjerg; G. Palapattu; J. Sønksen; E. Høgdall

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 1569-9056)

Published

2018

Language

EN