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Decreased Expression of Peroxisome Proliferator Activated Receptor Gamma in Cftr-/- Mice by Mario Ollero; Omer Junaidi; Munir M. Zaman; Iphigenia Tzameli; Adolfo A. Ferrando; Charlotte Andersson; Paola G. Blanco; Eldad Bialecki; Steven D. Freedman is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Decreased Expression of Peroxisome Proliferator Activated Receptor Gamma in Cftr-/- Mice about?
Some of the pathological manifestations of cystic fibrosis are in accordance with an impaired expression and/or activity of PPARgamma. We hypothesized that PPARgamma expression is altered in tissues lacking the normal cystic fibrosis transmembrane regulator protein (CFTR). PPARgamma mRNA levels were measured in colonic mucosa, ileal mucosa, adipose tissue, lung, and liver from wild-type and cftr-/- mice by quantitative RT-PCR. PPARgamma expression was decreased twofold in CFTR-regulated tissues (colon, ileum, and lung) from cftr-/- mice compared to wild-type littermates. In contrast, no differences were found in fat and liver. Immunohistochemical analysis of PPARgamma in ileum and colon revealed a predominantly nuclear localization in wild-type mucosal epithelial cells while tissues from cftr-/- mice showed a more diffuse, lower intensity labeling. A significant decrease in PPARgamma expression was confirmed in nuclear extracts of colon mucosa by Western blot analysis. In addition, binding of the PPARgamma/RXR heterodimer to an oligonucletotide containing a peroxisome proliferator responsive element (PPRE) was also decreased in colonic mucosa extracts from cftr-/- mice. Treatment o
Who reads Decreased Expression of Peroxisome Proliferator Activated Receptor Gamma in Cftr-/- Mice?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Mario Ollero; Omer Junaidi; Munir M. Zaman; Iphigenia Tzameli; Adolfo A. Ferrando; Charlotte Andersson; Paola G. Blanco; Eldad Bialecki; Steven D. Freedman
- Publisher
- John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0021-9541)
- Published
- 2004
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)