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Differential Regulation of Microglial Keratan Sulfate Immunoreactivity by Proinflammatory Cytokines and Colony-stimulating Factors by Sebastian Jander; Michael Schroeter; Johannes Fischer; Guido Stoll is a Neuroscience article available to read on EtoBox.

What is Differential Regulation of Microglial Keratan Sulfate Immunoreactivity by Proinflammatory Cytokines and Colony-stimulating Factors about?

Resident microglia of the rat CNS express a unique type of keratan sulfate immunoreactivity (KS-IR) that is lacking on peripheral monocytes/macrophages and associated with a so far unknown proteoglycan core protein. Microglial KS-IR is downregulated during T-cell-mediated autoimmune inflammation but largely preserved in degenerative lesion paradigms. This study addresses the role of cytokines and colony-stimulating factors in the regulation of microglial KS-IR. In vitro, ramified microglia in coculture with astrocytes, but not isolated microglia, constitutively expressed KS-IR under control conditions. In both culture paradigms, KS-IR was increased significantly by macrophage- (M-CSF) and granulocyte/macrophage colony-stimulating factors (GM-CSF), as well as tumor necrosis factor-alpha (TNF-alpha). By contrast, the Th1 cytokine interferon-gamma (IFN-gamma) downregulated KS-IR, both when applied alone or in combination with either GM-CSF, M-CSF, or TNF-alpha. In vivo, the intracerebroventricular administration of IFN-gamma, but not TNF-alpha, to healthy rats led to an almost complete disappearance of KS-IR from ramified brain microglia. Our data suggest that the expression of microg

Who reads Differential Regulation of Microglial Keratan Sulfate Immunoreactivity by Proinflammatory Cytokines and Colony-stimulating Factors?

Author

Sebastian Jander; Michael Schroeter; Johannes Fischer; Guido Stoll

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2000

Language

EN

Field

Neuroscience (Life Sciences)