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Multiple subcellular mRNA distribution patterns in neurons: A nonisotopic in situ hybridization analysis by Michele A. Paradies; Oswald Steward is a Neuroscience article available to read on EtoBox.

What is Multiple subcellular mRNA distribution patterns in neurons: A nonisotopic in situ hybridization analysis about?

Previous studies have established that most of the mRNAs that neurons express are localized in the cell body and very proximal dendrites, whereas a small subset of mRNAs is present at relatively high levels in dendrites. It is not clear, however, whether particular mRNAs have the same subcellular distribution in different types of neurons or whether different types of neurons sort mRNAs in different ways. The present study was undertaken to address these questions. Nonisotopic in situ hybridization techniques were used to define the subcellular localization of representative mRNAs including beta-tubulin, low-molecular-weight neurofilament protein (NF-68), high-molecular-weight microtubule-associated protein (MAP2), growth-associated protein 43 (F1/GAP43), the alpha subunit of calcium/calmodulin-dependent protein kinase II (alpha CaMII kinase), and poly (A+) mRNA. The mRNAs for beta-tubulin, neurofilament 68, and F1/GAP43 were restricted to the region of the cell body and very proximal dendrites in most neurons. In some neuron types, however, labeling for NF-68 extended for considerable distances into dendrites. In some neurons that express MAP2, the mRNA was present at the highest

Who reads Multiple subcellular mRNA distribution patterns in neurons: A nonisotopic in situ hybridization analysis?

Author

Michele A. Paradies; Oswald Steward

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0022-3034)

Published

1997

Language

EN

Field

Neuroscience (Life Sciences)