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CRISPR-Cas9 Gene Integration in Yeast by Aamir Rasool is a document available to read on EtoBox.

What is CRISPR-Cas9 Gene Integration in Yeast about?

The study presents CrEdit, a novel CRISPR/Cas9 mediated method for efficient multi-loci gene integration in Saccharomyces cerevisiae, addressing the challenges of low integration efficiencies and reliance on selection markers. CrEdit achieves near 100% efficiency for single gene integrations with short homology arms and demonstrates successful simultaneous integration of three genes for β-carotene production. This approach offers a flexible and cost-effective tool for genome engineering applications in meta

Author

Aamir Rasool

Language

EN