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Insights into RAG Evolution from the Identification of “Missing Link” Family A RAGL Transposons by Eliza C Martin; Lorlane Le Targa; Louis Tsakou-Ngouafo; Tzu-Pei Fan; Che-Yi Lin; Jianxiong Xiao; Ziwen Huang; Shaochun Yuan; Anlong Xu; Yi-Hsien Su; Andrei-Jose Petrescu; Pierre Pontarotti; David G Schatz is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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## Abstract A series of “molecular domestication” events are thought to have converted an invertebrate RAG-like (RAGL) transposase into the RAG1–RAG2 (RAG) recombinase, a critical enzyme for adaptive immunity in jawed vertebrates. The timing and order of these events are not well understood, in part because of a dearth of information regarding the invertebrate __RAGL-A__ transposon family. In contrast to the abundant and divergent __RAGL-B__ transposon family, __RAGL-A__ most closely resembles __RAG__ and is represented by a single orphan __RAG1-like__ (__RAG1L__) gene in the genome of the hemichordate __Ptychodera flava__ (__PflRAG1L-A__). Here, we provide evidence for the existence of complete __RAGL-A__ transposons in the genomes of __P. flava__ and several echinoderms. The predicted RAG1L-A and RAG2L-A proteins encoded by these transposons intermingle sequence features of jawed vertebrate RAG and RAGL-B transposases, leading to a prediction of DNA binding, catalytic, and transposition activities that are a hybrid of RAG and RAGL-B. Similarly, the terminal inverted repeats (TIRs) of the __RAGL-A__ transposons combine features of both __RAGL-B__ transposon TIRs and RAG recombinat

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Author

Eliza C Martin; Lorlane Le Targa; Louis Tsakou-Ngouafo; Tzu-Pei Fan; Che-Yi Lin; Jianxiong Xiao; Ziwen Huang; Shaochun Yuan; Anlong Xu; Yi-Hsien Su; Andrei-Jose Petrescu; Pierre Pontarotti; David G Schatz

Publisher

Oxford University Press (OUP)

Published

2023

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)