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Can I read Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers on EtoBox?
Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers by Gillian M. Beattie; Ana D. Lopez; Nathan Bucay; Andrew Hinton; Meri T. Firpo; Charles C. King; Alberto Hayek is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers about?
To date, all human embryonic stem cells (hESCs) available for research require unidentified soluble factors secreted from feeder layers to maintain the undifferentiated state and pluripotency. Activation of STAT3 by leukemia inhibitory factor is required to maintain "stemness" in mouse embryonic stem cells, but not in hESCs, suggesting the existence of alternate signaling pathways for self-renewal and pluripotency in human cells. Here we show that activin A is secreted by mouse embryonic feeder layers (mEFs) and that culture medium enriched with activin A is capable of maintaining hESCs in the undifferentiated state for >20 passages without the need for feeder layers, conditioned medium from mEFs, or STAT3 activation. hESCs retained both normal karyotype and markers of undifferentiated cells, including Oct-4, nanog, and TRA-1-60 and remained pluripotent, as shown by the in vivo formation of teratomas. Stem Cells 2005;23:489-495
Who reads Activin A Maintains Pluripotency of Human Embryonic Stem Cells in the Absence of Feeder Layers?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Gillian M. Beattie; Ana D. Lopez; Nathan Bucay; Andrew Hinton; Meri T. Firpo; Charles C. King; Alberto Hayek
- Publisher
- Oxford University Press (OUP)
- Published
- 2005
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)