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Microglial Precursors Derived from Mouse Embryonic Stem Cells by Isabella Napoli; Katrin Kierdorf; Harald Neumann is a Neuroscience article available to read on EtoBox.

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## Abstract Microglia are resident immune cells of the central nervous system. They can be directly isolated from the brain or from mixed postnatal glial cultures. Isolation of primary microglia is inefficient due to low yield. The cell line BV2 was used as a substitute for primary microglia, but BV2 are oncogenically transformed cells. Here, we established a protocol to generate microglial precursor lines from mouse embryonic stem (ES) cells. Microglial precursor cells were obtained from murine ES cells by differentiation of embryoid bodies to microglia within a mixed brain culture. Several independent ES cell‐derived microglial precursor (ESdM) lines were generated and characterized by flow cytometry, immunocytochemistry, and functional assays. All ESdM showed expression of IBa1, CD11b, CD45, F4/80, CD49d, and CD29, but were negative for cKit and CD34. Stimulation with interferon‐γ or lipopolysaccharide (LPS) demonstrated upregulation of proinflammatory cytokine gene transcription including nitric oxide synthase‐2, interleukin‐1β, and tumor necrosis factor‐α at levels comparable to primary microglia. The ESdM showed efficient and rapid phagocytosis of microsphere beads, which was

Who reads Microglial Precursors Derived from Mouse Embryonic Stem Cells?

Author

Isabella Napoli; Katrin Kierdorf; Harald Neumann

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley; Research Square (ISSN 0894-1491)

Published

2009

Language

EN

Field

Neuroscience (Life Sciences)