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Discovering the environmental potential of multi-family residential areas for nature-based solutions. A Central European cities perspective by Iwona Zwierzchowska; Dagmar Haase; Diana Dushkova is a Environmental Science article available to read on EtoBox.

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We designed a fluorescent peptide -magnetic nanoparticle conjugate that images E-selectin expression in mouse xenograft models of Lewis lung carcinoma (LLC) by fluorescence reflectance imaging. It was synthesized by attaching the E-selectin -binding peptide (ESBP; CDSDSDITWDQLWDLMK) to a CLIO(Cy5.5) nanoparticle to yield ESBP-CLIO(Cy5.5). Internalization by activated human umbilical vein endothelial cells (HUVECs) was rapid and mediated by E-selectin, indicated by the lack of uptake of nanoparticles bearing similar numbers of a scrambled peptide (Scram). To demonstrate the specificity of E-selectin targeting to ESBP-CLIO(Cy5.5) in vivo, we coinjected ESBP-CLIO (Cy5.5) and Scram-CLIO(Cy3.5) and demonstrated a high Cy5.5/Cy3.5 fluorescence ratio using the LLC. Histology showed that ESBP-CLIO was associated with tumor cells as well as endothelial cells, but fluorescenceactivated cell sorter analysis showed a far less expression of E-selectin on LLC than on HUVECs. Using immunohistochemistry, we demonstrated E-selectin expression in both endothelial cells and cancer cells in human prostate cancer specimens. We conclude that ESBP-CLIO (Cy5.5) is a useful probe for imaging E-selectin ass

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Author

Iwona Zwierzchowska; Dagmar Haase; Diana Dushkova

Publisher

Elsevier BV

Published

2021

Language

EN

Field

Environmental Science (Physical Sciences)