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The effects of storage on the retention of enzyme activity in cryostat sections. A quantitative histochemical study on rat liver by Wilma M. Frederiks; Ilse J. M. Ouwerkerk; Klazina S. Bosch; Frans Marx; Arnold Kooij; Cornelis J. F. Van Noorden is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.
What is The effects of storage on the retention of enzyme activity in cryostat sections. A quantitative histochemical study on rat liver about?
The effect of storage of unfixed cryostat sections from rat liver for 4 h, 24 h, 3 days and 7 days at -25 degrees C was studied on the activities of lactate dehydrogenase, glucose-6-phosphate dehydrogenase, xanthine oxidoreductase, glutamate dehydrogenase, succinate dehydrogenase (all demonstrated with tetrazolium salt procedures), glucose-6-phosphatase (cerium-diaminobenzidine method), 5'-nucleotidase (lead salt method), dipeptidyl peptidase II, acid phosphatase (both simultaneous azo coupling methods), D-amino acid oxidase (cerium-diaminobenzidine-cobalt-hydrogen peroxide procedure) and catalase (diaminobenzidine method). The effect of drying of the cryostat sections at room temperature for 5 and 60 min was investigated as well. The enzyme activities were quantified by cytophotometric measurements of test and control reactions. The test minus control reaction was taken as a measure for specific enzyme activity. It was found that the activities of all the enzymes investigated, with one exception, were affected neither by storage of the cryostat sections at -25 degrees C for up to 7 days, nor by drying of the sections at room temperature for up to 60 min. The exception was xanthine
Who reads The effects of storage on the retention of enzyme activity in cryostat sections. A quantitative histochemical study on rat liver?
It is typically read by researchers, students, and practitioners in Biochemistry, Genetics and Molecular Biology.
- Author
- Wilma M. Frederiks; Ilse J. M. Ouwerkerk; Klazina S. Bosch; Frans Marx; Arnold Kooij; Cornelis J. F. Van Noorden
- Publisher
- Springer; Springer-Verlag; Kluwer Academic Publishers; Springer Science and Business Media LLC (ISSN 0018-2214)
- Published
- 1993
- Language
- EN
- Field
- Biochemistry, Genetics and Molecular Biology (Life Sciences)