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Type I procollagen synthesis is regulated by steroids and related hormones in human osteosarcoma cells by Anitta Mahonen; Arja Jukkola; Leila Risteli; Juha Risteli; Pekka H. Mäenpää is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Changes in the synthesis of type I collagen, the major extracellular matrix component of skin and bone, are associated with normal growth, tissue repair processes, and several pathological conditions. Expression of the COL 1A1 gene is regulated by transcriptional and post-transcriptional mechanisms. However, the hormonal regulation of type I collagen synthesis in human bone has not been well characterized. We have studied the influence of calcitriol, dexamethasone, retinoic acid, and estradiol on the COL 1A1 gene expression by determining the secretion of the C-terminal propeptide (PICP) and the levels of ␣1(I) procollagen mRNA in cultured human MG-63 and SaOs-2 osteoblast-like osteosarcoma cells. Similar experiments were also performed with respect to expression of the nuclear proto-oncogenes, c-fos and c-jun, in MG-63 cells. In MG-63 cells, calcitriol stimulated the synthesis and secretion of PICP. The ␣1(I) procollagen mRNA level was elevated with no effect on message stability, indicating a transcriptional mechanism of regulation. In contrast, dexamethasone treatment was accompanied by an accelerated rate of ␣1(I) procollagen mRNA turnover, observed as decreased amounts of the

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Author

Anitta Mahonen; Arja Jukkola; Leila Risteli; Juha Risteli; Pekka H. Mäenpää

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 0730-2312)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)