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Marked Dependence on Growth State of Backup Pathways of NHEJ by Frank Windhofer; Wenqi Wu; Minli Wang; Satyendra K. Singh; Janapriya Saha; Bustanur Rosidi; George Iliakis is a Medicine article available to read on EtoBox.

What is Marked Dependence on Growth State of Backup Pathways of NHEJ about?

Purpose: Backup pathways of nonhomologous end joining (B-NHEJ) enable cells to repair DNA double-strand breaks (DSBs) when DNA-PK-dependent NHEJ (D-NHEJ) is compromised. Recent evidence implicates growth signaling in the regulation of D-NHEJ. This study was intended to determine whether the ability to repair DSBs by B-NHEJ also depends on growth state. Methods and Materials: LIG4 -/-and wild type (WT) mouse embryo fibroblasts (MEFs) were used. Repair of DSBs was measured by pulsed-field agarose gel electrophoresis. G1 cells were selected by centrifugal elutriation. A plasmid assay was used to measure DNA end-joining activity in whole cell extracts. Results: Wild-type MEFs efficiently repaired DSBs by D-NHEJ in either the exponential or plateau phase of growth. Because of their defect in ligase IV, which compromises D-NHEJ, LIG4 -/-MEFs showed reduced repair capacity but were slowly able to rejoin a large proportion of DSBs via B-NHEJ. B-NHEJ was markedly reduced in the plateau phase of growth or at high radiation doses. Elutriated G1 cells from exponentially growing or plateauphase LIG4 -/-cultures showed a response similar to nonelutriated cells, ruling out that the effect simply

Who reads Marked Dependence on Growth State of Backup Pathways of NHEJ?

Author

Frank Windhofer; Wenqi Wu; Minli Wang; Satyendra K. Singh; Janapriya Saha; Bustanur Rosidi; George Iliakis

Publisher

Elsevier Science; Elsevier ; Elsevier BV (ISSN 0360-3016)

Published

2007

Language

EN

Field

Medicine (Health Sciences)