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Developmental expression and localization of the catalytic subunit of protein phosphatase 2A in rat lung by Chun Xue; Felice Heller; Roger A. Johns; Allen D. Everett is a Biochemistry, Genetics and Molecular Biology article available to read on EtoBox.

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Protein phosphatase type-2A (PP2A) is a highly conserved serine/threonine phosphatase known to play a key role in cell proliferation and differentiation in vitro, but the role of PP2A in mammalian embryogenesis remains unexplored. No particular information exists as to the tissue or cell specific expression of PP2A or the relevance of PP2A expression to mammalian development in vivo. To examine expression of PP2A during mammalian lung development, we studied fetal rats from day 14 of gestation (the lung bud is formed on day 12 of gestation) to parturition. Western analysis with a specific PP2A catalytic subunit antibody identified a single 36 kDa protein, with protein levels two-fold higher in the 17 and 19 day embryonic lung as compared to the adult. With in situ hybridization and immunohistochemistry, both mRNA and protein for PP2A were localized equally to the epithelial lining of the embryonic lung airway and the surrounding mesenchyme in the 14 day embryonic lung. With maturation of the lung, PP2A becomes highly expressed in respiratory epithelium. The highest level of expression was in the earliest developing airways with columnar epithelium (the pseudoglandular stage, 15-18

Who reads Developmental expression and localization of the catalytic subunit of protein phosphatase 2A in rat lung?

Author

Chun Xue; Felice Heller; Roger A. Johns; Allen D. Everett

Publisher

John Wiley and Sons; Wiley (John Wiley & Sons); John Wiley & Sons Inc.; Wiley (ISSN 1058-8388)

Published

1998

Language

EN

Field

Biochemistry, Genetics and Molecular Biology (Life Sciences)